Bats are the only indigenous mammals on Reunion Island, with one of the three species, Mormopterus francoismoutoui, being endemic, and another, Pteropus niger, endangered. The endemic bat is highly adapted to urban areas and coexists in proximity to humans, living in large colonies in rooftops of houses, schools, and churches, and also under bridges. These bats hold considerable importance for Reunion Island’s natural heritage, but at the same time, they carry diseases and leave dejections that can cause olfactory discomfort and respiratory problems. As conservation and management measures hold profound implications on both human and bat populations, we need to foster a proper understanding of stakeholder perspectives and find common ground. We applied Q methodology to explore stakeholder discourses of bats on Reunion Island, focusing on aspects of conservation, management, and public health. Our combined quantitative and qualitative analyses revealed three principal discourses. The human-oriented discourse advocates public education and addressing myths and misconceptions to protect bats. The nature-oriented discourse prioritizes the protection of natural habitats to conserve bats. The public health and policy-oriented discourse seeks to adapt bat conservation and management policies to ensure public health safety. All stakeholders agreed that there is a lack of awareness among local media, which at times diffuses sensational and erroneous information, and that as well there is a need to integrate research output into public outreach efforts to garner interest in bats. These results can aid in contextualizing conservation actions and policies and could potentially provide sustainable management solutions to human-bat coexistence on Reunion Island.